Table 2

Phosphorylated peptides from BCR/ABL-expressing cells treated with STI571

ProteinNCBI GI no.Peptide sequencePO4 siteKnown PO4 site (ref.)Top scansite hits (24)0 hr1.5 hr3 hr
32d p210
 ABL125135LMTGDTpYTAHAGAKY393Y393 (43)NonexNA
 ABL125135LMTGDTpYpTAHAGAKY393, T394Y393 (43)Y393:None,Y394:NonexNA
 ABL125135LpYVSSESRY185NonexxNA
 ABL125135pYSLTVAVKY264Y264 (44)NonexNA
 ABL125135NKPTVpYGVSPNYDKY226Y226 (43)B-3.3,X-3.4,HH-2.5xNA
 ABL125135VpYELMRY469AA-0.25,GG-2.8,II-3.3xNA
 BCR11038641HQDGLPYIDDpSPSSpSPHLSSKS459, S463S459:M-4.0,R-0.56,S463:G-1.3,H-1.6,R-0.32xNA
 CAS-L8567380LpYQVPNSQAASRY91B-1.5,C-1.0,J-0.56xxNA
 CAS-L8567380DVpYDVPPSHSTQGVpYDIPPS SVKY176, Y188Y176:C-0.14,J-0.01,Z-0.04 Y188:C-0.20,J-0.01,Z-0.03xNA
 CBL115857IKPSSSANAIpYSLAARY655Y655 (39)B-2.2xxNA
 CBL115857LPPGEQGEpSEEDTEpYMTPTS RPVGVQKPEPKS675, Y681Y681 (45) (Y700)S675:E-3.6,F-0.40,K-1.8 Y681:J-0.08,W-0.04,Z-0.06xxNA
 DYRK1A6015042IYQpYIQSRY321Y321 (46)NonexNA
 SHEP17305329AGESpYTHIRY273NonexNA
 SHP-26755228VpYENVGLMQQQRY584Y584 (47)O-0.55,Y-1.9,JJ-1.9xNA
k562
 ABL125135LMTGDTpYTAHAGAKY393Y393 (43)Nonexx
 ABL125135LMTGDTpYpTAHAGAKY393, T394Y393 (43)Y393:None,Y394:Nonexx
 ABL125135LpYVSSESRY185Nonexx
 ABL125135LGGGQpYGEVYEGVWKY253N-4.0,P-4.0,X-0.20x
 ABL125135LGGGQYGEVpYEGVWKY257X-0.32,Y-0.65,II-0.83x
 Activated p21cdc42Hs18553914pYATPQVIQAPGPRY827J-4.6,W-3.0x
 Activated p21cdc42Hs423137VSSTHpYpYLLPERPSYLERY912, Y913Y912:BB-1.2,GG-2.1,HH-0.04 Y913:C-3.9,J-3.4,DD-1.3x
 BCR11038641PFpYVNVEFHHERY177Y177 (30)N-2.7,Q-0.03,BB-3.2x
 BCR11038641HQDGLPYIDDpSPSSpSPHLSSKS459, S463S459:M-4.0,R-0.56,S463:G-1.3,H-1.6,R-0.32xx
 BCR11038641RLpTWPRT310A-2.4,D-1.9,EE-0.55xx
 BCR11038641NSLETLLpYKPVDRY644Nonex
 BCR11038641LASQLGVpYRY591Nonex
 CBL115855IKPSSpSANAIpYSLAARS669, Y674Y674 (39)Y674:B-2.2,S669:Nonex
 CBL115855IKPSSSANAIpYSLAARY674Y674 (39)B-2.2x
 Cortactin4885205LPSSPVpYEDAASFKY421Y421 (48)O-1.6,Y-1.3,AA-2.0x
 DNABP14557447ELEEIpYMLPRY1068B-0.37,W-0.66,DD-1.3x
 e3b12245671HNSTTSSTSSGGpYRY305Nonex
 GAB14503851SYpSHDVLPKS266D-0.82,E-3.6,I-4.2x
 GAB14503851APSASVDSSLpYNLPRY259C-2.8,J-0.76,Z-2.1xxx
 GAB218105042HNTEFRDSTpYDLPRY266C-0.65,J-0.56,Z-0.22x
 GAGE-34503881STpYYWPRPRY10B-0.59,C-1.3,J-2.9x
 p56dok4503359GQEGEpYAVPFDAVARY299B-0.04,W-0.66,X-0.67x
 p62dok4503357SHNSALpYSQVQKY449P-3.0x
 plakophilin 44505843NNYALNTTATpYAEPpYRPIQYRY478, Y482Y478:B-0.02,C-0.52,W-1.0,Y482:Nonex
 SHC284403ELFDDPSpYVNVQNLDKY317Y317 (49)L-0.47,Q-0.01,BB-0.33x
 SHIP-24755142TLSEVDpYAPAGPARY1135W-4.3xxx
 SHIP-24755142GLPSDpYGR(PLSFPPPR)Y1162Y1162 (39)B-0.60,J-0.96,C-2.8xx
 SHIP-24755142DTpYAWHKY671Nonex
 SHIP-24755142LpYEWISIDKDEAGAKY886P-1.1,W-1.5,GG-0.52x
 SHIP-24755142NSFNNPApYYVLEGVPHQLLPP EPPpSPARY986, S994Y986 (50)Y986:L-0.20,N-1.7,FF-0.19,S994:Nonex
 SYK1174527EALPMDTEVpYESPpYADPEEIR PKY348, Y352Y348, Y352 (51)Y348:C-0.65,W-0.07,Z-0.22 Y352:C-1.9,W-0.18,BB-3.2x
  • Times are after STI571 treatment. Scansite motifs are described in Table 1. NA, time points not analyzed in the experiments.