Table 1. Surface membrane proteins sensitive to NAC treatment
Spot no. Protein name NCBI accession no. PBM score Z score Percent sequence coverage No. of matching peptides MALDI MS identification methods
1 NMMHC-IIA gi189036 171 2.43 24 27 PMF
2 VLA-4 gi4504749 - 0.12 7 6 PSD
3 β-actin gi4501885 82 2.1 37 13 PMF
4 MLC gi576 96 2.43 46 9 PMF
  • Spot nos. refer to Fig. 3. PBM and Z scores are the probability scores obtained with mascot and profound programs with PMF data. PSD analysis was used as confirmation when PMF data did not give significant scores (PBM score <63 and Z score <1.65). MALDI, matrix-assisted laser desorption/ionization.