Table 1

Proteins differentially accumulated in AHL-treated roots as percent of controls

  • * Proteins are grouped by putative function, recognizing that some functions may belong in more than one group. 

  • This protein shows homology to a ripening induced protein of unknown function but is also inducible in soybean by the soybean-cyst nematode and so may be defense-related in roots. 

  • The M. truncatula (M.t.) tentative clusters (TCs) against which the proteins were identified. These can be accessed at www.tigr.org/tdb/tgi/mtgi/

  • § TOG indicates the Tentative Ortholog Group into which the M. truncatula TCs have been classified by The Institute for Genomic Research (TIGR). Access is through www.tigr.org/tdb/tgi/mtgi/. n/a indicates that no TOG has been assigned to this TC. 

  • The observed Mr and pl for each M. truncatula protein were determined from its position on the gels relative to known marker proteins. 

  • ** −Cl2 = 3-oxo-C12-HL; C16 = 3-oxo-C16:1-HL. 

  • ‡‡ Relative accumulations are given in percent of the control level and are averages of results from three individual control gels and three individual treatment gels. Accumulation levels are also indicated by color code: yellow, a new protein not detected in controls; red, significantly increased; tan, nonsignificantly increased; pale green, nonsignificantly reduced; dark green, significantly reduced; blue, reduced below detection. P values for statistical significance of difference from control levels ranged from <0.001 to <0.005 but were <0.002 for >90% of the proteins listed. 

  • †† Only two gels were examined for proteins from roots exposed to the lowest concentration (10 nM) of 3-oxo-C12-HL. 

  • §§ Estimated concentration; see Materials and Methods