Table 1

Strains and plasmids used in this study

Strain/plasmidRelevant genotype/commentsSource/ref.
Plasmids
 pKD20λ-RED (gam, bet, exo), bla, araC, rep101ts2
 pCP20FLP-recombination plasmid: flp, bla, cat, rep101ts 11
 pIJ666Source of cat gene for pIJ790 24
 pOJ436Source of ApraRoriT sequence for pIJ773 25
 pIJ790λ-RED (gam, bet, exo), cat, araC, rep101tsThis study
 pIJ773aac(3)IV (ApraR) + oriTThis study
 pIJ778aadA from Ω-fragment (SpecR, StrepR) + oriTThis study
 pIJ779aadA from Ω-fragment (SpecR, StrepR)This study
 pIJ780vph from Ω-fragment (VioR) + oriTThis study
 pIJ781vph from Ω-fragment (VioR)This study
 Supercos-1 bla, neo, cos Stratagene
 pUZ8002tra, neo, RP4 23
 pUB307neo, RP4 30
 pSETΩ aadA, oriT, attP, int 31
E. coli
 BW25113K12 derivative: ΔaraBAD, ΔrhaBAD2
 ET12567 dam, dcm, hsdS, cat, tet 29
 BT340DH5α/pCP20 11
S. coelicolor
 M145SCP1, SCP21
 KF62cyc1∷Tn4560 insertion at aa position 75This study
 J3001Δcyc2: aa 2–725 replaced with pIJ773 cassetteThis study
 J3002cyc1∷Tn4560cyc2This study
 J3003Δcyc2scar: aa 2–725 replaced with scar sequenceThis study
 J3004cyc2ΔN: aa 6–340 replaced with scar sequenceThis study
 J3005cyc2ΔC: aa 380–721 replaced with scar sequenceThis study
 J3006cyc1∷Tn4560,cyc2ΔNThis study
 J3007cyc1∷Tn4560,cyc2ΔCThis study