Table 1

Fatty acid species in triglyceride

CellsFA supplementFA, nmol/mg protein
16:016:118:018:118:2
CHONone2.59  ± 0.040.33  ± 0.020.69  ± 0.031.59  ± 0.230.26  ± 0.08
CHOPalm19.56  ± 0.920.26  ± 0.041.02  ± 0.141.31  ± 0.140.17  ± 0.05
CHOPalm/oleate84.39  ± 0.363.74  ± 0.794.13  ± 0.7420.77  ± 1.640.43  ± 0.18
25RANone7.72  ± 1.340.81  ± 0.001.53  ± 0.116.87  ± 0.690.58  ± 0.16
25RAPalm39.79  ± 4.441.94  ± 0.062.14  ± 0.104.74  ± 0.830.29  ± 0.04
SCDNone1.88  ± 0.370.41  ± 0.020.30  ± 0.052.40  ± 0.290.20  ± 0.30
SCDPalm73.31  ± 9.5013.10  ± 2.332.35  ± 0.4122.75  ± 3.171.05  ± 0.24
  • CHO, 25RA, and SCD-overexpressing CHO cells (SCD) were incubated in media supplemented where indicated with 500 μM palmitate (palm) or 500 μM palmitate plus 200 μM oleate (palm/oleate) for 6 h. The amount of palmitate (16:0), palmitoleate (16:1), stearate (18:0), oleate (18:1), and linoleate (18:2) present in triglyceride pool is reported and represents the mean of three independent experiments ± SE.