Table 2

Deuterated fatty acids in triglyceride fractions of SCD and CHO cells

Cell typeDeuterated FA, nmol/mg protein
d16:0d16:1d18:0d18:1
CHO14.07  ± 1.660.52  ± 0.131.37  ± 0.070.58  ± 0.06
SCD54.63  ± 2.637.79  ± 0.353.06  ± 0.3111.01  ± 0.58
  • CHO and SCD-overexpressing cells (SCD) were incubated in media supplemented with 500 μM deuterated palmitate for 6 h. Incorporation of deuterated palmitate (d16:0), deuterated palmitoleate (d16:1), deuterated stearate (d18:0), and deuterated oleate (d18:1) in the triglyceride fraction is reported and represents the mean of three independent experiments ± SE.