Table 1. RNAi-mediated reduction of target gene mRNA levels
Target gene mRNA reduction, %
rab11b 96 ± 1
rab8a 93 ± 4
VIP17 92 ± 2
Annexin 13 91 ± 2
Caveolin-1 90 ± 3
TI-VAMP 90 ± 4
MAL2 88 ± 3
Annexin 2 81 ± 4
VIP36 81 ± 5
rab11a 77 ± 6
Annexin 13b 74 ± 4
KIFC3 73 ± 4
Syntaxin 3 71 ± 2
  • Target gene mRNA levels were determined by quantitative RT-PCR. Levels in knockdown cells are given as percent mRNA reduction relative to the levels in control cells transduced with empty RVH1-puro. Standard deviations are from at least three independent experiments in each case. Note that annexin 13a cannot be targeted individually because annexin 13b comprises the entire annexin 13a sequence. The annexin 13 knockdown therefore affects both isoforms.