Table 1.

Enzymatic parameters of AdK variants

AdK variantkcat, s−1KM, μM
Wild-type340 ± 3053 ± 17
AdKcc,ox1.5 ± 0.118 ± 8
AdKcc,red26 ± 237 ± 12
AdK G56C31 ± 146 ± 2
AdK T163C195 ± 550 ± 5