Table 1. Cell—cell movement of MP:GFP fluorescence in epidermal cells of infected leaves
hpi
Virus Plant line 14—15 16—17 18—19 20—21 22—23 24—25 36—37
TMV- Sx 0/22 (0)* 0/46 (0) 2/67 (3) 15/121 (12) 25/57 (44) 41/60 (68) 27/33 (82)
MP:GFP N.b. 0/25 (0) 12/28 (43) 12/30 (40) 19/35 (54) 35/44 (80) 85/113 (75)
NN 14/75 (19) 14/57 (25) 42/61 (69) 71/126 (56) 119/130 (92)
277 (+MP) 0/6 (0) 3/16 (19) 11/17 (59) 20/29 (69) 13/21 (62)
ToMV Sx 0/24 (0) 0/65 (0) 1/52 (2) 6/54 (11) 14/34 (41) 44/63 (70) 17/19 (90)
MP:GFP 277 (+MP) 5/30 (17) 15/36 (42) 23/33 (70) 30/39 (77) 37/51 (72) 31/33 (94)
3646 (+CP) 0/24 (0) 0/30 (0) 2/32 (6) 8/22 (36) 21/38 (55) 39/52 (75)
  • The fraction of infection centers in which MP:GFP has spread from primary infected cells to adjacent cells. Plants: Sx, N tabacum Samsun; N.b., N benthamiana; NN, N tabacum Xanthi NN; 277, transgenic N tabacum Xanthi nn producing the TMV movement protein; 3646, transgenic N. tabacum Xanthi nn producing TMV-CP.

  • * Total number of infection centers where MP:GFP moved/total number of infection centers observed × 100. Percents are in parentheses

  • Infection by ToMV MP:GFP; ToMV that is closely related to TMV. These data indicated that results of ToMV MP:GFP are similar to that of TMV