Table 1. Inhibition of recombinant protease activity and parasite multiplication
Protease activity, μM*
Parasite multiplication, μM
Compound Falcipain-2 Falcipain-3 3D7 W2 HB3
E-64 0.012 0.032 2.9 2.6 2.1
E-64d 4.9 6.3 1.9 0.5 1.8
YA29 86.4 >400 9.4 8.8 21.1
Pepstatin ND ND 8.3 6.8 6.3
Chloroquine ND ND 0.01 >0.10 0.009
  • ND, not determined.

  • * All values are mean IC50, based on three replicates for each inhibitor, with all experiments performed with the same concentration of enzyme.

  • Mean IC50 values were determined from two experiments, each done in duplicate.