Table 1.

Parameters derived by fitting of the observed association and dissociation time courses of the sT1 TCR-H-2Kd-pPbSAB interaction at 25°C shown in Fig. 1A to a two-exponential model

[sT1], μM 20 10 5 2.5 1.3
Phase Association Dissociation Association Dissociation Association Dissociation Association Dissociation Association Dissociation
a0 1 0.09 1 0.10 1 0.12 1 0.16 1 0.17
a1 −0.90 0.76 −0.84 0.72 −0.74 0.64 −0.68 0.58 −0.63 0.54
k1, 10−1·s−1 2.6 1.4 2.6 1.5 2.6 2.6 1.9 2.6 2.2
a2 −0.10 0.15 −0.16 0.18 −0.26 0.24 −0.32 0.26 −0.37 0.28
k2, 10−2·s−1 2.0 1.1 2.2 1.4 2.1 1.6 2.0 1.8 1.9 1.8
  • The total amplitudes of each time course (a0 + a1 + a2) were normalized to 1.