Table 1. Tissue eicosanoid level
6-keto PGF1α PGF2α PGE2 PGD2 15-HETE 12-HETE 5-HETE
Normal 0 1.65 ± 0.98 12.77 ± 3.73 0.20 ± 0.29 3.67 ± 2.00 3.83 ± 1.92 0.50 ± 0.21
COX-2 24.87 ± 0.67 45.31 ± 26.87 173.64 ± 11.62 8.34 ± 5.29 21.85 ± 0.08 19.62 ± 5.65 1.37 ± 0.96
NM + indomethacin 0 0 1.17 ± 1.75 1.45 ± 1.75 3.82 ± 1.49 25.720 ± 11.56 0.52 ± 0.09
COX-2 + indomethacin 1.26 ± 2.18 0 1.49 ± 2.58 2.51 ± 2.58 5.25 ± 2.11 33.13 ± 15.46 0.62 ± 0.17
  • Mammary glands were excised, and eicosanoid levels were quantified by the liquid chromotographic-electrospray MS procedures, as described. Data represent values from two to three mammary glands isolated from different animals (ng per 30 mg of wet weight).