Table 4. Relationship between the frequency of integration into a homologous site in the genome and the length of the homologous sequence
Strain Length of homology, bp Exp. no. Bla-resist PFP-resist Homologous integration, %
Wild type 50 1 22 0
2 29 1
Total 51 1 2
mus-51 50 1 30 0
2 29 1
Total 59 1 2
mus-52 50 1 29 0
2 30 0
Total 59 0 0
Wild type 100 1 20 0
2 30 1
Total 50 1 2
mus-51 100 1 8 2
2 13 0
Total 21 2 10
mus-52 100 1 14 0
2 32 2
Total 46 2 4
Wild type 500 1 40 4
2 40 3
Total 80 7 9
mus-51 500 1 40 35
2 39 37
Total 79 72 91
mus-52 500 1 30 28
2 39 36
Total 69 64 93
Wild type 1,000 1 9 3
2 22 7
3 40 6
4 40 7
Total 111 23 21
mus-51 1,000 1 4 4
2 8 8
3 10 10
4 8 8
Total 30 30 100
mus-52 1,000 1 31 31
2 19 19
Total 50 50 100
  • Bla-resist, blasticidin resistance; PFP-resist, PFP resistance.