Table 1. Ngn3-induced genes in mPAC L20 cells
GenBank accession no. Protein name Fold increase* SEM Confirm
Transcription
NM_010919 Nkx2.2 16.3 4.2 C
NM_008814 PDX-1 14.5 7.4 C
MMU76208 Ngn3 12.5 3.5 C
AB038696 Olig-1 12.0 5.0 C
Y09166 Neurogenin1 11.3 2.3 C
NM_007897 Ebf1 10.3 5.3 NT
NM_010895 NeuroD2 7.3 1.5 C
X74134 Coup TF1 7.0 2.4 C
L14610 RZR beta 6.7 2.1 NT
MMU28068 NeuroD1 6.2 0.8 C
S79041 Gsh2 6.1 2.9 C
AF153046 Hb9 5.5 1.6 NT
S80555 RAR beta 4.9 1.1 NT
BG074244 Nsbp1 4.7 1.0 NT
AF260236 Hes-6 4.5 0.7 C
XM_123529 Lhx4 4.4 1.0 NT
NM_009385 Nkx2.1 4.3 0.8 NT
AF086757 NSCL1 4.0 1.2 NT
NM_010133 Engrailed 1 3.8 1.0 NT
AB031040 Lhx6.1a 3.4 0.9 NT
U79550 Slug 3.2 0.7 NT
U67841 Dlx6 2.7 0.2 C
AF202039 Nkx2.4 2.6 0.6 NT
NM_021459 Isl-1 2.6 0.1 C
Signaling
NM_010950 Numb-Like 10.7 2.4 C
NM_011183 Presenilin2 9.5 2.7 NC
NM_017218 ErbB3 8.4 1.4 NT
X80903 Delta 1 7.9 4.1 C
NM_008943 Presenilin 1 6.1 1.5 NT
MMU04710 IGF-II receptor 5.0 0.8 NT
BC010770 Somatostatin 4.4 1.1 C
D32210 Notch2 4.3 1.7 C
M33385 TrkB 4.2 1.2 NC
L20336 Dopamine receptor 1a 4.1 0.9 NT
Z48746 FGF8 3.0 0.4 NT
NM_010491 IAPP 2.6 0.4 C
X93581 TrkA 2.6 0.4 C
Cell organization
NM_007667 Cadherin 8 7.2 2.1 NT
M31131 Cadherin N 5.9 1.5 C
NM_009152 Sema3a 4.4 0.8 NC
AF041083 Robo 1 4.3 0.9 NT
NM_008737 Neuropilin-1 4.0 0.7 C
NM_013657 Sema3c 3.6 0.7 NT
BG069465 Antigen CD63 3.6 0.5 NT
NM_010111 EphrinB2 3.0 0.2 NT
BG085134 Antigen CD24a 2.6 0.3 NT
BG076893 RhoB 2.6 0.5 NT
Protein metabolism
BG078497 Cathepsin L 4.7 1.7 C
BG076444 p162 subunit of elF3 2.5 0.5 NT
DNA synthesis
BG069854 DNA repair XRCC1 3.0 0.6 NT
  • SEM, standard error of the mean.

  • * mPAC L20 cells were treated with AdCMV-βgal or AdCMV-ngn3, and RNA was extracted 48 h later. Data are means of fold induction by Ngn3 relative to β-galactosidase from four hybridizations using RNA from three independent experiments. All genes induced ≥2.5-fold are shown.

  • Confirmation of the microarray data was performed for a subset of genes by using RT-PCR. C, upregulation was confirmed in at least two of three independent experiments; NC, not confirmed; NT, not tested.

  • These genes were spotted more than once on the array, and the fold induction shown is the mean of all spots for that gene.