Table 1. Genes showing ≥5-fold induction by both 1 mM GSNO and 1 mM NaNO2
GSNO
NaNO2
Gene b no. 0.1 mM 1 mM 0.1 mM 1 mM Function
norV b2710 173 85 75 78 Flavorubredoxin
norW b2711 338 98 169 52 Flavorubredoxin reductase
hmpA b2552 70 37 53 31 Flavohemoglobin
ytfE b4209 38 29 21 50 Homology to NO-dependent regulators
soxS b4062 2 27 2 36 Regulator of soxRS regulon
yftA b2597 3 19 2 22 Homology to σ54 modulators
fes b0585 11 15 14 17 Enterochelin esterase
yhjX b3547 2 14 3 9 Homology to oxalate/formate antiporters
nrdH b2673 17 14 18 10 Glutaredoxin-like protein
nrdI b2674 18 8 19 5 Probably involved in ribonucleotide reductase function
ybaE b0445 1 14 1 9 Putative ABC-type transporter
ychH b1205 1 11 1 9 Putative membrane protein
hofF b3327 6 11 7 11 Putative general secretory pathway protein
ygbA b2732 5 10 4 8 Conserved hypothetical protein
tdcB b3117 2 10 2 7 Threonine dehydratase
uspA b1895 1 9 1 6 Universal stress protein A
hycI b2717 15 8 14 6 Hydrogenase 3 maturation protease
ybhG b0795 2 8 2 7 Homology to HlyD-family secretion proteins
sufA b1684 6 7 7 7 Homology to iron-binding protein IscA
yadG b0127 7 7 6 10 Putative ABC-type transporter
fhuF b4367 9 7 9 10 Ferric iron reductase protein
yqeB b2875 1 6 1 5 Homology to xanthine and CO dehydrogenases maturation factors
yhaK b3106 1 6 1 25 Homology to pirin-related proteins
yjjM b4357 1 6 1 9 Homology to transcriptional regulators
yfiD b2579 1 6 1 6 Homology to formate acetyltransferases
ybaR b0484 2 6 4 7 Homology to copper-transporting P-type ATPase
asnA b3744 4 6 4 5 Asparagine synthetase A
deoC b4381 1 6 1 5 Deoxyribose-phosphate aldolase
ilvC b3774 2 6 2 8 Ketol-acid reductoisomerase
ybiJ b0802 2 6 2 44 Conserved hypothetical protein
ascF b2715 2 6 1 6 Phosphotransferase system (PTS) system enzyme IIABC
ycgT b1200 2 5 1 5 Homology to dihydroxyacetone kinase
adhE b1241 2 5 2 6 Aldehyde-alcohol dehydrogenase
ycfR b1112 2 5 2 17 Conserved hypothetical protein
  • Columns show average of induction ratios for two independent RNA samples isolated from MG1655 cells left untreated or exposed to 0.1 or 1 mM concentrations of GSNO or NaNO2 for 5 min. For all induction ratios ≥2, the P values are <0.05. The norVW and nrdHI genes, which are contained within operons, are grouped together. For most genes within operons, all genes show induction, though the induction ratios decrease for the downstream genes. Descriptions of function are taken from http://genolist.pasteur.fr/Colibri and/or www.ncbi.nlm.nih.gov/BLAST.