Table 1. Statistical parameters for PKA and PTP-1B
% Phosphorylation Z′ Z factor S/N % CV S/B SW
Evaluation of the performance of the PKA assay
100 0.94 na 59 3.2 7.5 80
50 0.92 51 2.6
25 0.92 48 1.0
10 0.84 26 2.1
5 0.84 23 0.6
Evaluation of the performance of the PTP-1B assay
100 0.87 na 27 2.6 2.5 45
50 0.85 33 1.6
  • Na, not applicable; S/N, Signal to Noise; % CV, coefficient of variation; S/B, signal to background; SW, signal window.