Table 2. The GCR rates in the GCR mutator strains were reduced by different mutations in mitotic checkpoint genes
bub1Δ bub3Δ mad2Δ mad3Δ bub2Δ
rfc5-1 ND 0.6 (<0.01) 7.0 (0.01) 43.9 (0.13) 2.6 (<0.01)
mec1Δ* 20.6 (0.03) 0.8 (<0.01) 91.6 (0.35) 43.5 (0.31) 65.7 (0.94)
dun1Δ 12.4 (0.02) 1.0 (<0.01) 35.1 (0.21) 1.0 (<0.01) 122.4 (0.57)
mre11Δ 8.2 (<0.01) 11.3 (<0.01) 20.9 (<0.01) 28.3 (<0.01) 1.2 (<0.01)
rfa1-t33 44.4 (<0.01) 21.5 (<0.01) 85.2 (0.70) 17.4 (<0.01) 14.4 (<0.01)
rad27Δ 108.3 (0.33) 70.9 (0.52) 83.2 (0.24) 58.3 (0.19) 100.0 (0.43)
rad52Δ 120.0 (0.26) 86.0 (0.35) 74.0 (0.73) 91.0 (0.97) 25.0 (0.94)
pif1-m2 141.6 (0.85) 19.7 (0.01) 16.1 (<0.01) 13.1 (0.01) 2.2 (<0.01)
  • The number given is the fold increase of the GCR rate of the double mutant indicated by the intersection of the mutations listed along the top row and the left column relative to wild type expressed as a percentage of the fold increase of the GCR rate of the single mutant indicated along the left column relative to wild type (calculated from the data of Table 1) and is a measure of the extent of suppression observed. Numbers in parentheses are the P values (Mann—Whitney test) calculated for the significance of GCR rate reduction by the addition of each mitotic checkpoint gene mutation. ND, not determined.

  • * mec1Δ strains also have a sml1Δ mutation.