Table 1. Percent relative concentration of extracellular compounds in culture supernatants of mutant strains vs. wild-type isogenic PA14
% of PA14
Compound mvfR-pqsA-pqsB-mvfR- compl. lasR-
HHQ 0 0 0 1017 ± 247 250 ± 11
HHQ N-oxide 0 0 0 200 ± 41 45 ± 8
PQS 0 0 0 247 ± 65 23 ± 3
HNQ 0 0 0 502 ± 106 551 ± 262
HNQ N-oxide 0 0 0 286 ± 52 41 ± 3
diHNQ 0 0 0 156 ± 49 19 ± 3
AA 0 458 ± 221 188 ± 102 0 6 ± 1
Pyocyanin <10 <10 <10 177 ± 26 NT
  • Cells were cultivated in LB medium at 37°C to OD600 = 4.0-4.5. Data are averages of triplicate experiments ± SD. diHNQ, 3,4-Dihydroxy-2-nonylquinoline; NT, not tested. mvfR compl., the mutant strain complemented with pDN18mvfR.