Table 3. Split-ubiquitin system interaction between RGT2 and MTH1 or STD1
Mean β-gal activity, U ± SD (fold activation over vector alone)
Rgt2-Cub-PLV (pBM4546)
Rgt2Δbox-Cub-PLV (pBM4548)
NubG plasmid 2% galactose 4% glucose 2% galactose 4% glucose
pNubG-HA-X (vector) 663 ± 76 140 ± 16 509 ± 134 217 ± 7
NubG-Mth1 (pBM4549) 17,300 ± 568 (26 X) 291 ± 11 (2.1 X) 402 ± 30 (0.8 X) 217 ± 45 (1.0 X)
NubG-Mth18ST (pBM4563) 14,700 ± 452 (22 X) 1,818 ± 35 (13 X) 536 ± 28 (1.0 X) 200 ± 15 (0.9 X)
NubG-Std1 (pBM4550) 2,750 ± 299 (4.1 X) 202 ± 4 (1.4 X) 330 ± 58 (0.7 X) 183 ± 5 (0.8 X)
NubG-Std19SA (pBM4551) 8,650 ± 2030 (13 X) 883 ± 196 (6.3 X) 343 ± 6 (0.7 X) 185 ± 43 (0.9 X)