Table 1. Activation of a reporter gene by nucleoporins in Δnup84 or Δgcr1 cells
lexA fusion Δnup84 Δgcr1
Nup84 74.2 ± 1.4
Nup145N 9.5 ± 0.1 19.4 ± 0.4
Nup145C 18.4 ± 0.1 22.2 ± 0.2
hNup98 19.6 ± 0.3 52.2 ± 0.9
Nup85 58.1 ± 1.4 8.7 ± 0.1
Nup120 7.2 ± 0.1 25.6 ± 0.7
Nup133 <0.1* 6.4 ± 0.1
Sec13 <0.1* 30.2 ± 0.3
Nup53 <0.1* 28.9 ± 0.1
Gcr1 19.8 ± 0.1
Gcr2 18.1 ± 0.5 50.2 ± 1.5
Gcn4 102.7 ± 1.4 180.3 ± 4.0
Swi4 57.3 ± 1.7 130.4 ± 7.9
Ssn8 32.4 ± 0.5 13.3 ± 0.8
Sin4 93.2 ± 0.2 11.9 ± 0.1
  • Activity of lexA fusions was determined in either a Δnup84 or a Δgcr1 strain; the percent of β-gal activity in these strains relative to that in WT cells (see Fig. 4) is shown ± SD. —, Not determined.

  • * No detectable activity.