Cells | Crest cell expression, % |
---|---|
Phox2b^{+} neurons | 28 ± 10 |
X-gal^{+} cells | 10 ± 2 |
Predicted Phox2b^{+} X-gal^{+} neurons* | 2.9 |
Actual Phox2b^{+} X-gal^{+} neurons | 1.6 ± 0.5† |
The percentage of Phox2b^{+}, X-gal^{+}, or Phox2b^{+} X-gal^{+} double-positive cells was first measured in each neural tube explant as (no. marker cells/total DAPI cells) × 100%. The mean of this percentage was then calculated for all explants (n = 5). Five to seven fields (×40) in the Phox2b rich area of the explants were randomly selected per explant. BMP2 was used at 50 ng/ml, and cells were cultured for 5 days.
↵ * The predicted percentage of Phox2b^{+} X-gal^{+} neurons is calculated as the product of the numbers in the first two rows.
↵ † The mean percentage of the actual contribution to A neurons is significantly different from the predicted contribution (assuming there is no bias, P = 0.03 from five explants).