Table 3. Mass spectrometry analysis of HPLC fractions
Fraction Mass [M + H]+ Sequence Positions* Putative epitope(s)*
1 1196.63 FYTPKTRRE 49–57 49–57
3 1115.58 RGFFYTPKT 46–54
1115.58 GFFYTPKTR 47–55
1271.68 GFFYTPKTRR 47–56
1400.72 GFFYTPKTRRE 47–57 49–57
1764.85 VEALYLVCGERGFFY 36–50 41–50
4 975.45 GERGFFYT 44–51 44–51
1078.45 CGERGFFYT 43–51 44–51
1177.52 VCGERGFFYT 42–51 42–51, 44–51
1177.59 HLCGSHLVEAL 29–39 30–39
1355.65 EALYLVCGERGF 37–48 39–48
  • * N- and C-terminal amino acids in proinsulin