Table 5. Binding of proinsulin peptides to HLA class I
Binding* %
Peptides Sequence HLA AM§ 10–4 M 10–6 M Type
30–39 LCGSHLVEAL A2 + 0 0 0
34–42 HLVEALYLV A2 ++ 58 37 I
38–46 ALYLVCGER A3 ++ 57 47 I
ALYLVCGER A11 ++ 149 64 H
39–48 LYLVCGERGF A24 ++ 49 100 H
41–50 LVCGERGFFY A1 + 54 35 I
LVCGERGFFY A3 + 68 49 I
LVCGERGFFY A11 + 90 89 H
41–51 LVCGERGFFYT A2 + 0 0 0
42–51 VCGERGFFYT A1 + 32 37 I
VCGERGFFYT A2 + 25 20 I
VCGERGFFYT B8 + 43 20 I
VCGERGFFYT B18 + 4 60 H
44–51 GERGFFYT A1 + 58 10 L
GERGFFYT B8 + 41 16 L
45–53 ERGFFYTPK A3 + 15 42 I
49–57 FYTPKTRRE B8 + 10 70 H
50–58 YTPKTRREA B8 + 0 0 0
51–58 TPKTRREA B8 ++ 0 0 0
51–61 TPKTRREAEDL B8 + 25 8 L
  • * % of binding = tested peptide/viral reference peptide

  • N- and C- terminal amino acids in proinsulin

  • Anchoring residues are underlined

  • § Anchoring motifs: ++ = strict, + = tolerated

  • Type of binder; L, low binder; I, intermediate binder; H, high binder; 0, no binding