Table 1. Changes in residues belonging to clusters from multiple sequence alignment
Cluster Site Polarity Charge
I A184S/C +/−
I193V +
N199S
A203S +
A/V207S +
A218S/G +
A220S +
T221A
D224G/S −(−)
II Q378R +(+)
T/Q389A
T/A489G/S +
M410L +
V414L
S422A
T425V
I/V428M
I/L430V +
T/S442L/F
III P488L/V
T492L
R/H497P −(+)
L502F
F512V +
V528A +
W536G/S +
G541A
  • Each entry refers to a residue in consensus electrogenic transporters that has been changed in the NaPi-IIc sequence. Numbering refers to mouse NaPi-IIa sequence. +/− indicates increase/decrease in polarity or charge, respectively, according to a hydrophobicity scale (25).