Table 1. S. aureus strains used in this study
bursa aurealis insertion/mutation
Phenotype
Strain Genotype nt position AA position Orientation EsxA secretion EsxB secretion
Newman Clinical isolate Yes Yes
ΦNΞ187-01 esxA24 24 8 NA No No
ΦNΞ09349 esaA::erm 1110 370 (+) Yes Yes
ΦNΞ03742 essA::erm 121 41 (-) No No
ΦNΞ 13152 esaB::erm 43 15 (+) Yes Yes
ΦNΞ 02411 essB::erm 199 67 (+) No No
ΦNΞ04464 essC::erm 792 264 (-) No No
ΦNΞ02191 essC::erm 1854 618 (-) No No
ΦNΞ02832 essC::erm 2161 721 (+) Low Low
ΦNΞ13038 essC::erm 3835 1,279 (+) Yes Yes
ΦNΞ07912 esxB::erm 2 1 (+) No No
  • All Phoenix strains (ΦNΞ) are isogenic to S. aureus Newman (21) and are part of our laboratory collection (20). In strain esxA24, a stop codon replaces the coding codon at position 8. AA, amino acid; erm, erythromycin resistance gene; nt, nucleotide.