Table 1. Quantification of APC mutations in plasma
Patient no. Sex/age, yr Site Dukes' stage (tumor node metastasis stage) Diameter of lesion, cm Mutation identified in primary tumor (codon) Fragments per ml of plasma No. of fragments analyzed Percentage of mutant fragments, %
1 M/50 Ascending colon Adenoma 3.0 C4348T (1450) 2,600 2,350 0.002
2 M/67 Descending colon Adenoma 2.5 C4285T (1429) 5,080 5,080 0.001
3 M/54 Rectum Adenoma 4.0 G3856T (1286) 4,150 4,150 0.002
4 F/82 Rectum Adenoma 3.0 4147–4148insA (1383) 1,350 1,350 0.001
5 F/65 Rectum Adenoma 1.0 C4067G (1356) 4,260 4,260 0.001
6 F/71 Ascending colon Adenoma 4.0 G3856T (1286) 4,150 4,150 0.001
7 M/68 Cecum Adenoma 6.5 C4285T (1429) 4,760 4,760 0.003
8 M/93 Ascending colon Adenoma 0.8 A4345T (1449) 4,320 4,320 0.001
9 F/78 Ascending colon Adenoma 3.0 C4216T (1406) 28,570 28,570 0.001
10 F/59 Sigmoid colon Adenoma 5.0 4661–4662insA (1554) 2,160 2,160 0.002
11 F/73 Ascending colon Adenoma 5.0 C4348T (1450) 8,000 8,000 0.02
Median/mean 4,300/6,300 0.02*
Mutant plasma samples per samples analyzed 1/11 (9)
12 F/81 Sigmoid colon A (T2N0M0) 4.0 G4189T (1397) 7,900 12,000 0.01
13 F/75 Sigmoid colon A (T2N0M0) 2.5 3927–3931del AAAGA (1309) 2,160 2,160 0.001
14 M/60 Sigmoid colon A (T2N0M0) 3.0 3927–3931del AAAGA (1309) 4,600 6,900 0.04
15 M/79 Right colic flexure A (T2N0M0) 3.0 4470delT (1490) 4,600 3,696 0.03
16 M/70 Ileocecal A (T2N0M0) 2.5 4481delA (1494) 6,200 3,105 0.07
17 F/68 Ascending colon A (T2N0M0) 3.5 C4348T (1450) 2,170 2,170 0.001
18 F/66 Sigmoid colon A (T1N0M0) 2.5 3927–3931del AAAGA (1309) 1,920 1,920 0.001
19 M/68 Rectum A (T2N0M0) 5.5 C3907T (1303) 2,300 1,170 0.12
Median/mean 3,500/4,000 0.04/0.04*
Mutant plasma samples per samples analyzed 5/8 (63)
20 F/65 Cecum B (T3N0M0) 3.5 G4396T (1466) 5,300 5,300 0.002
21 M/71 Sigmoid colon B (T3N0M0) 3.0 C4348T (1450) 2,100 1,863 0.19
22 M/37 Descending colon B (T4N0M0) 10.0 C4330T (1444) 5,400 4,887 1.28
23 M/64 Sigmoid colon B (T3N0M0) 6.5 C4099T (1367) 3,810 3,810 0.001
24 M/72 Sigmoid colon B (T3N0M0) 3.0 C4012T (1338) 4,800 4,800 0.03
25 F/82 Hepatic flexure B (T3N0M0) 4.0 C4099T (1367) 3,840 3,840 1.46
26 M/83 Ascending colon B (T3N0M0) 6.0 4470delT (1490) 1,600 1,404 1.75
27 M/61 Sigmoid colon B (T3N0M0) 4.0 4260–4261delCA (1420) 4,200 4,200 0.001
Median/mean 4,000/3,900 1.28/0.94*
Mutant plasma samples per samples analyzed 5/8 (63)
28 F/83 Ascending colon D (T3N2M1) 5.0 4661–4662insA (1554) 230,000 24,857 5.6
29 M/55 Sigmoid colon D (T3N0M1) 3.0 G3925T (1309) 69,600 1,636 27.4
30 F/33 Descending colon D (T4N1M1) 5.0 C4067A (1356) 18,000 491 10.5
31 M/64 Sigmoid colon D (T4N2M1) 6.0 T4161A (1387) 26,000 975 1.9
32 M/56 Rectum D (T3N2M1) 3.0 4468–4469delCA (1490) 103,200 1,187 18.9
33 F/60 Rectum D (T3N2M1) 4.0 4059–4060insT (1354) 8,400 850 2.0
Median/mean 47,800/75,900 8.05/11.05*
Mutant plasma samples per samples analyzed 6/6 (100)
  • * Calculated only for samples in which the percentage of mutant fragments was significantly higher than in control samples (i.e., >0.003%; printed in boldface). M, male; F, female