Table 1. UVR8 and HY5 regulate a range of genes concerned with UV protection
wt UV-B versus uvr8 UV-B
wt UV-B versus hy5 UV-B
Probe-set ID Gene Name RPscore FDR FCrma RPscore FDR FCrma
258321_at At3g22840 Early light-induced protein (ELIP1) 1.7 0 -156.71 40.27 0 -5
245306_at At4g14690 Early light-induced protein (ELIP2) 2.36 0 -92.41 279.78 1.77 -2.59
252123_at At3g51240 Flavanone 3-hydroxylase (F3H) 3.26 0 -63.22 46.69 0 -4.71
253496_at At4g31870 Glutathione peroxidase, putative 4.71 0 -43.67
250207_at At5g13930 Chalcone synthase (CHS) 5.5 0 -34.76 5.08 0 -9.96
249063_at At5g44110 ABC transporter family protein 6.42 0 -28.16 230.02 1.22 -2.86
250533_at At5g08640 Flavonol synthase 1 (FLS1) 6.85 0 -26.52 2.35 0 -13.32
249769_at At5g24120 RNA polymerase subunit SigE (sigE) 9.6 0 -20.23
251020_at At5g02270 ABC transporter family protein 9.96 0 -20.08 401.58 3.14 -2.45
247463_at At5g62210 Embryo-specific protein-related 11.13 0 -19.05 1.66 0 -15.7
246966_at At5g24850 Cryptochrome dash (CRYD) 13.66 0 -15.39 5.23 0 -9.7
245560_at At4g15480 UDP-glucoronosyl/UDP-glucosyl transferase family protein 15.21 0 -14.56 18.61 0 -6.33
251658_at At3g57020 Strictosidine synthase family protein 16.73 0.07 -13.45
252010_at At3g52740 Expressed protein 21.41 0.07 -11.51 323.82 2.37 -2.66
251827_at At3g55120 Chalcone isomerase (CHI) 23.11 0.06 -10.84 20.88 0 -6.08
249191_at At5g42760 O-methyltransferase N terminus domain-containing protein 23.81 0.06 -11.05
251727_at At3g56290 Expressed protein 24.83 0.06 -10.23
253039_at At4g37760 Squalene monooxygenase, putative 25.89 0.05 -10.12
246468_at At5g17050 Flavonoid 3-O-glucosyl transferase 26.33 0.05 -9.76
250420_at At5g11260 bZIP protein HY5 (HY5) 27.24 0.05 -9.93 58.93 0.03 -4.56
248049_at At5g56090 Cytochrome oxidase assembly family protein 30.44 0.05 -9.05 83.53 0.12 -3.67
250083_at At5g17220 Glutathione S-transferase, AtGST12/TT19 31.93 0.04 -8.64 16.03 0 -6.77
249215_at At5g42800 Dihydroflavonol 4-reductase (DFR) 32.04 0.04 -8.7 56.27 0.04 -4.52
263122_at At1g78510 Solanesyl diphosphate synthase (SPS) 33.57 0.04 -8.5
259537_at At1g12370 Type II CPD photolyase PHR1 (PHR1) 35.67 0.04 -8.03 184.55 0.75 -3.03
253943_at At4g27030 Expressed protein 36.84 0.04 -8.09
260955_at At1g06000 UDP-glucoronosyl/UDP-glucosyl transferase family protein 37.27 0.03 -8.12 98.76 0.17 -3.98
250794_at At5g05270 Chalcone-flavanone isomerase family protein 38.01 0.03 -8.25 15.62 0 -6.55
249798_at At5g23730 Transducin family protein/WD-40 repeat family protein 38.51 0.03 -7.73 332.09 2.46 -2.5
262626_at At1g06430 FtsH protease, FtsH8 41.46 0.03 -7.46
252661_at At3g44450 Expressed protein 42.44 0.03 -7.55
262705_at At1g16260 Protein kinase family protein 47.38 0.06 -6.86
253879_s_at At4g27570 Glycosyltransferase family protein 54.76 0.05 -6.35 42.2 0 -4.96
248347_at At5g52250 Transducin family protein/WD-40 repeat family protein 56 0.05 -6.31
255594_at At4g01660 ABC1 family protein 56.86 0.05 -6.24
258349_at At3g17610 bZIP transcription factor HY5-like protein (HYH) 58.07 0.04 -6.01
265634_at At2g25530 AFG1-like ATPase family protein 61.67 0.09 -5.95 185.64 0.74 -2.96
260072_at At1g73650 Expressed protein 66.05 0.1 -5.56
264752_at At1g23010 Multi-copper oxidase type I family protein 69.4 0.1 -5.7 104.77 0.2 -3.65
  • The genes listed are induced by UV-B in wild type but show much reduced UV-B induction in uvr8-1 (left columns) and, in many cases, hy5-1 (right columns). The data-set comparisons used for gene identification are explained in Materials and Methods, and the complete data for 72 genes are presented in Table 2, which is published as supporting information on the PNAS web site. The microarray data were analyzed by the Rank Products method (22); RPscore is a measure of differential expression, FDR indicates the expected percentage of false positives, and FCrma is a measure of the fold change in expression. The Probe-set ID is the Affymetrix probe-set identifier.