Table 1. ATPase activity sensitivity of FoF1 to DCCD inhibition
Samples Residual activity +DCCD, %
Membrane vesicles 25
Reconstituted vesicles 20
FoF1 15
  • The samples were pretreated with 50 μM DCCD for 30 min and subjected to the assays. Membrane vesicles were prepared from Escherichia coli cells expressing FoF1 from thermophilic Bacillus PS3. Purified FoF1 was incorporated into reconstituted vesicles. ATPase activity was measured in the same solution used for rotation observation. Other experimental details are described in Materials and Methods.