Table 2. C57BI/6 mice immunized with uis4REP - sporozoites are completely protected against a challenge with WT sporozoites
Immunization with uis4REP- sporozoites Boosts (days after immunization/no. of sporozoites) No. protected/no. challenged (prepatency)
50,000 1st (14/25,000), 2nd (28/25,000) 8/8 (no infection)*
None None 0/5 (day 3)
10,000 1st (14/10,000), 2nd (28/10,000) 8/8 (no infection)*
None None 0/5 (day 3)
10,000 1st (14/10,000), 2nd (29/10,000) 0/4 (day 2)
  • * Immunized mice were challenged with 50,000 WT Pb sporozoites at day 38 after immunization. Mice were from the same age group, and sporozoites were from the same mosquito batch. Blood smears were evaluated up to day 50 after challenge

  • Naive control mice were from the same age group and were challenged with 50,000 WT Pb sporozoites

  • Immunized mice were challenged with 10,000 blood stage parasites at day 34 after immunization