Table 2.

Effect of LPA1 expression by tumor cells on LPA-induced cytokine secretion

Cell linesIL-6IL-8GM-CSFGROαMCP-1
SKBRr-34 ± 00001 ± 1
SKBr/LPA1#3.1454 ± 64572 ± 421,033 ± 133577 ± 62281 ± 40
SKBr/LPA1#4.1491 ± 54578 ± 911,013 ± 144576 ± 72282 ± 38
BO2/GFP467 ± 71987 ± 102,313 ± 2671,161 ± 55407 ± 45
BO2/GFP-Sb1#9524 ± 471,048 ± 1822,241 ± 2281,142 ± 124382 ± 113
BO2/GFP-Sb1#10570 ± 251,079 ± 682,219 ± 3681,117 ± 72380 ± 132
BO2/GFP-SiLPA1#122236 ± 45491 ± 7665 ± 14310 ± 176 ± 4
BO2/GFP-SiLPA1#123217 ± 13446 ± 16597 ± 16014 ± 45 ± 4
BO2/GFP-SiLPA1#132209 ± 6439 ± 11679 ± 1399 ± 47 ± 4
  • The specific production of cytokines secreted in the culture media from cells treated with 1-oleoyl LPA (1 μM) for 48 h was quantified by ELISA. Data are expressed as mean ± SD (in pg/ml per 106 cells) of three replicates and are representative of two separate experiments.