Table 1.

Real-time PCR confirmation of cytokinin-regulated microarray results

Gene nameAt numberWild type + vs. − BA
crf1,2,5 + vs. − BA
crf2,3,6 + vs. − BA
MicroarrayRT-PCRMicroarrayRT-PCRMicroarrayRT-PCR
Zinc Finger B-boxAt1g685203.12.8 ± 0.31.01.3 ± 0.11.11.1 ± 0.1
NAM AtNAC6At4g274102.72.7 ± 0.21.30.7 ± 0.11.41.0 ± 0.1
Speckled POZAt3g483603.52.7 ± 0.31.10.8 ± 0.10.91.0 ± 0.5
Senescene Assoc. P.At1g538853.92.7 ± 0.31.31.7 ± 0.12.01.5 ± 0.3
ARR5At3g481007.314.3 ± 0.43.226.6 ± 1.15.025.4 ± 0.9
ARR7At1g190503.69.5 ± 0.35.715.1 ± 0.65.512.1 ± 0.4
  • Four genes regulated by cytokinin in wild type, but not in CRF mutants, were examined by using real-time PCR, as well as two type-A ARR genes and show similar trends to the microarrays in Fig. 4A.