Table 1.

MS analysis of purified apo- and holo-proteins

Protein*MALDI-MS
ESI-FT-ICR-MS
4′ Ppant elimination
Theo. [M + H]+Exper. [M + H]+Theo. avg massExp. avg massMoietyTheo. [M + H]+Exp. [M + H]+
ZmaD
    apo-ZmaD12,23912,23912,238.712,238.3
    holo-ZmaD12,57912,57912,579.012,579.3
    glyceryl-ZmaD12,66712,668 12,667.112,667.4 Glyceryl447.120447.121
12,670
    HM-ZmaD12,681ND12,681.112,681.3HM461.099461.099
    glycolyl-ZmaD12,63712,63612,637.112,637.4Glycolyl417.109417.110
ZmaH
    apo-ZmaH11,60911,60511,609.111,608.8
    holo-ZmaH11,94911,94611,949.411,949.1
    seryl-ZmaH12,03712,038 § 12,036.512,036.8Seryl446.136446.136
12,036
    AM-ZmaH12,051ND
    glycyl-ZmaH12,00712,00712,006.512,006.8Glycyl416.125416.124
  • Masses are in daltons. Theo., theoretical; Exp., experimental; ND, not detected.

  • *The mass of ZmaD and ZmaH derivatives is calculated after removal of the first methionine.

  • The mass of glyceryl-ZmaD detected when 3-PG was the starting substrate.

  • The mass of glyceryl-ZmaD detected when glyceraldehyde-3-phosphate was the starting substrate.

  • §The mass of seryl-ZmaH detected in the ZmaJ, holo-ZmaH reaction.

  • The mass of seryl-ZmaH detected in the ZmaJ, holo-ZmaH, ZmaG, and ZmaI reaction.