Table 1.

Effect of NLS and PKA mutations on Maf1 function

MutationsPol III repression*Glycerol 37°C Growth SGal-His ade2-1 color § Location
−rap+rap
WT28++++N + CN
maf1Δ112+++++
6SA26++++N > CN
6SE28++++C > NN
6SAΔCtNLS42++++(+)+N > CN
6SEΔCtNLS91++++++++CC > N
ΔNtNLS35++++CC > N
ΔNtCtNLS101++++++++CC
ΔCtNLS64++++++++CN
  • *Residual transcription after 90 min of rapamycin treatment expressed as a percentage of the untreated WT level (from Fig. 4). Data is representative of multiple experiments that generate an SD of 3–8%. An examination of unstressed transcription for many Maf1 mutants found no significant changes compared with WT or the maf1Δ strain.

  • Glycerol phenotype: + represents each 10-fold dilution at which cells grew on SGly-Trp at 37°C.

  • tgm-silencing phenotype: + represents each 10-fold dilution at which cells grew on SGal-Ura-Trp-His at 30°C.

  • §Antisuppression: −, cream color; +, light pink; ++, pink; +++, red on SGal-Ura-Trp media.

  • Localization: Cytoplasmic (C) and nuclear signal (N) after 60 mins with (+) or without (−) rapamycin treatment. Qualitative changes in distribution are expressed relative to WT.