Table 1.

Lineage distribution and B-lymphoid in vitro colony-forming cell activity of TEL-AML1 progenitor cells

Zebrafish lineLineage distribution by FACS, no. of cells (×104) (%)
B cell colonies
ErythroidMyeloidLymphoidProgenitornCells harvested per well (×103)
Experiment 1
    Wild-type108 ± 17.3 (56.9 ± 9.1)38.4 ± 9.8 (20.9 ± 5.1)17.2 ± 7.3 (9.6 ± 3.8)8.7 ± 5.8 (4.8 ± 3.1)38 ± 9140
    XEF-TA123 ± 28.3 (68.2 ± 15.7)30.0 ± 7.1 (16.7 ± 3.9)5.9 ± 3.8 (3.3 ± 2.1)30.2 ± 8.3 (14.2 ± 4.6)* 3 ± 2* 18
    XEF-EGFP-TA108 ± 15.9 (59.9 ± 8.8)38.4 ± 6.3 (18.9 ± 3.5)14.7 ± 8.8 (8.2 ± 4.8)20.5 ± 9.3 (11.4 ± 5.2)* 7 ± 4* 43
    ZBA-EGFP-TA106 ± 10.3 (58.7 ± 5.7)16.0 ± 7.4 (8.9 ± 4.1)6.8 ± 4.2 (3.8 ± 2.3)41.9 ± 16.3 (23.3 ± 9.0)* 14 ± 9* 67
Experiment 2
    RAG2-EGFP-TA110 ± 12.9 (58.1 ± 7.2)32.9 ± 6.0 (18.3 ± 3.3)16.2 ± 6.3 (9.0 ± 3.5)10.2 ± 6.1 (5.4 ± 3.4)246 ± 231,211
    RAG2-EGFP106 ± 10.3 (59.2 ± 5.7)40.7 ± 5.2 (22.6 ± 2.8)15.8 ± 5.5 (8.8 ± 3.0)7.5 ± 4.3 (4.3 ± 2.4)229 ± 281,362
  • Total cell numbers and mean percentage of cells corresponding to erythroid, myeloid, lymphoid, and progenitor compartments were analyzed by FACS. Clonable B cell in 103 kidney marrow progenitors of wild-type and TEL-AML1 transgenic fish, and in the EGFP-positive (lymphoid progenitor enriched) fraction of marrow progenitors from RAG2-EGFP-TA and RAG2-EGFP control fish. Data are presented as the mean ± SD of 12 replicates from each transgenic line done in two independent experiments.

  • *Significantly higher number of progenitors or lower number of colonies, compared to wild-type or control cells.