Table 2.

ZFN-induced mutations

AlterationLong PCR products*
QQR, short PCR*
QQRNw WT lig-4
Fill-in
    4 bp25 (4) 6 (4)89 (17)0
    3 bp2 (2)110 (8)0
    2 bp1 (1)03 (3)0
    1 bp2 (2)000
    Complex2 (2)03 (2)0
Deletions
    <10 bp2 (2)17 (14)11 (11)7 (7)
    >10 bp8 (7)27 (17)08 (8)
Substitutions1 (1)3 (3)11 (10)61 (51)
  • *Long PCR products (from WT) were 1,006 bp for QQR and 752 bp for Nw; short QQR PCR products were 207 bp.

  • Sequences of these mutations are presented in Tables 4, 5, and 6, which are published as supporting information on the PNAS web site.

  • The numbers of alterations in each category are shown. The numbers in parentheses represent independent observations that are either different sequences or the same sequence recovered from different worms. The numbers of worms in each category are QQR-long, 4; Nw, 4; QQR-short WT, 18; QQR-short lig-4, 21.