Table 3.

Sequences of junctions formed in the germ line

Injected DNAGCTACGTAATACGACTCACTAGTGGGCACGATGCATTATGCTGAGTGATCACCCGTCTAGAGCTTCCCATGGTGACGTCACCGGTTCTAGGGTACCACTGCAGTGGCCAAGA
Products from WT AG --- --CTT (3)
AGATC ---TT
t GTGGGCAGATC -----g C
C----- -GCTT
AGA -- -GCTT
AGA-- AGCTT
A---- AGCTT
Products from lig-4 AG --- --CTT (2)
AGA-- ----TCC (2)
g GA-- ----TCC
CA---- ---------T
ACGT------------------------- -----------------CA
AGA-- ---------------------------CT
  • Substrate ends generated by BglII and HindIII digestion are shown in the top section. Symbols for deletions, insertions, substitutions and microhomologies are as in Table 1.