Table 2.

Term development of mouse embryos developed from the oocytes fertilized by injection of intact, immobilized, lysolecithin-treated and Triton X-100-treated mouse spermatozoa

Exp.Sperm injectedTotal no. of zygotes cultured (no. of reps.)No. (%) of zygotes developed to two-cellNo. of two-cells transferred (no. of recipients)No. (%) of live normal offspring at term
AIntact108 (7)102 (94)102 (9)41 (40.2)*
BIsolated heads142 (10)137 (96)137 (12)80 (58.3)
CLysolecithin-treated, individually123 (7)123 (100)123 (9)88 (71.5)
DLysolecithin-treated, as a group158 (7)149 (94)149 (13)70 (46.9)§
ETriton X-100-treated, individually118 (7)115 (97)98 (7)58 (59.2)
FTriton X-100-treated, as a group120 (9)118 (98)118 (11)43 (36.4)
  • Experiment (Exp.) A, a motile spermatozoon with intact head and tail was injected into each oocyte; B, only the head was injected after separation of the head and tail by piezo pulses; C and E, spermatozoa were individually treated for 1 min with 0.02% LL or 0.02% Triton X-100 and washed in Hepes-CZB containing 12% PVP for 1 min before injection of a single sperm head isolated from the tail by piezo pulses; D and F, ≈105 spermatozoa were treated for 1 min with 0.02% LL or 0.02% Triton X-100, washed by centrifugation for 3 min, and left in Hepes-CZB for 5–60 min before injection of a single sperm head isolated from the tail by piezo pulses. ∗ vs. †, P < 0.01; † vs. ‡, P < 0.01; ‡ vs. §, P < 0.01; ¶ vs. ‖, P < 0.01.