Table 2. Incidence of within-species barcode clusters showing correlated biological differences and their impact on estimates of intraspecific sequence divergence in three families of Lepidoptera
Mean barcode divergences, %
Family No. of morphological species No. of species with barcode clusters* % species with barcode clusters Morphological species Morphological species + barcode-detected species P
Hesperiidae 348 3 0.86 0.17 0.16 0.52
Sphingidae 107 4 3.74 0.43 0.35 0.16
Saturniidae 66 6 9.09 0.46 0.19 0.05
  • * Number of morphologically defined species composed of two or more barcode clusters with correlated biological differences.

  • Distances are calculated by using the Kimura two-parameter model of base substitution (23).

  • Calculated by using a paired t test.