Table 2.

Transfer of PAPI-1 between P. aeruginosa strains

Donor strain (marker)*Recipient strain (marker)*Transfer conditionsTransfer frequency
Plate matingND
PA14 140495 (GmR)PAO1CbR lacZ (CbR)Plate mating with heat shock6.6 × 10−7
Liquid mating6.6 × 10−5
Liquid mating with heat shock5.4 × 10−4
Plate mating3.1 × 10−7
PAO1 PAPI-1 (GmR)PAO1Tn1150 (TcR)Plate mating with heat shock3.5 × 10−7
Liquid mating4.5 × 10−6
  • *Antibiotic resistance: GmR, gentamicin resistance; CbR, carbenicillin resistance; TcR, tetracycline resistance.

  • ND, not detected.