Table 1.

Functional analysis of int and soj on PAPI-1 excision by PCR amplifications

Strains or mutants*Primer pairs
4542F + intF2 (junction between PA4542 and PAPI-1)sojR + 4541F (junction between PA4541 and PAPI-1)intF2 +sojR (circular PAPI-1)4542F + 4541F (empty attB site)976F + PAPI-1R (junction between PA0976 and PAPI-1)sojR167 + PAPI-2R (junction between PAPI-1 and PAPI-2)
PA14++++++
PA14Δint ++
PA14Δint pint ++++++
PA14Δint vector++
4542F + intFsojR2 +4541FintF + sojR2 4542F + 4541F976F + PAPI-1RsojR167 + PAPI-2R
PA14++++++
PA14Δsoj +
PA14psoj ++++++
PA14psojΔsoj +++++
PA14 vector++++++
  • “+” indicates a positive PCR amplification, whereas “−” indicates no PCR products. The gel images of PCR amplification are presented in SI Fig. 4. The chromosomal locations of primers are described in Figs. 1A and 2A.

  • *A detailed description of each mutant is listed in SI Table 3. pint and psoj are cloned int and soj on plasmid pPSV35, respectively. Vector is plasmid pPSV35.

  • Because primer sites intF and sojR are not present in mutant strains PA14Δint and PA14Δ soj, respectively, primer intF2, located upstream of int, and primer sojR2, located downstream of soj, are used.

  • Primer sojR167 is not present in mutant strain PA14psojΔsoj.