Table 2.

Antigenic analysis of H5N1 influenza viruses from different regions in Asia by HA inhibition titer

VirusSublineage*mAb to Ck/PA/1370/83
mAb to Ck/HK/YU22/02
mAb to VNM/1203/04
Antisera
CP25CP58CP176/263C87C68H1110H4D215A31203/04MS/04437.4/99
Ck/PA/1370/83Reference 12,800 12,800 12,800 12,80012,80012,80012,80012,8001608080
Gs/HK/437.10/993,20012,80012,80080012,80012,80012,80012,8001,280< 1280
Dk/HK/2986.1/001,6006,4001,6006,40012,80012,80012,80012,8001,280<640
Ck/HK/YU22/02200<200 6,400 1,600 1,600 12,800 12,800<80640
Dk/HN/5806/03HN<1,60012,80012,80080012,80012,800<<80320
Ck/HN/999/05<20012,8006,4001,6006,40012,800<<8080
Dk/YN/6445/03YN<<2003,200400800<200<160320
Ck/YN/115/04<2004006,4008001,600400400<80320
Ck/YN/447/05<1,6001,6001,600<400<<<<<
Ck/Indonesia/BL/03IDN<<80012,8001,60012,800<800<320640
Dk/Indonesia/MS/04<<40012,8001,6003,200<400< 160 640
Ck/Wajo/BBVM/05<<400<4001600<<<160320
Vietnam/1194/04VTM400200<12,8006,400<12,80012,8002,560<640
Vietnam/1203/04800400<6,4006,400<12,800 12,800 1,280 <1,280
Thailand/1(Kan-1)/04<<<12,80012,800<12,80012,8005,120<1,280
Dk/Vietnam/11/0440012,800<6,4003,20080012,80012,8001,280<640
Dk/Vietnam/S654/05<<<800400<1,600200160<160
Dk/Vietnam/568/05VNM2<<<<<<<<<<<
HK/212/03GD80020020012,80012,80080012,80012,8001603202,560
Gs/GX/345/05Mixed<<<6,400<200<200<<<
Ck/ST/810/05<<<800<<<<<<<
MDk/JX/1653/05MB400800200<3,2003,2006,4006,400<<640
MDk/JX/2136/05<80012,80012,80040012,800200200<<160
BH goose/QH/65/05<80012,80012,80040012,800<<<<<
  • <, lowest dilution tested: 1:100 for monoclonal antibody titres, 1:40 for ferret and chicken anti-sera. 437.4/99, Gs/HK/437.4/99; 1203/04, VNM/1203/04; GD; Guangdong; HN, Hunan; IDN, Indonesia; MB, migratory bird; MS/04, Dk/IDN/MS/04; VNM2, recent Vietnam introduction; VTM, Vietnam/Thailand/Malaysia; YN, Yunnan. Underlined numbers indicate titers to prototype viruses.

  • *Viruses were selected from each of the sub-sublineages as defined by phylogenetic analysis of the HA gene (Fig. 2A).