Table 1.

The Aeh1 NrdA-a/NrdA-b/NrdB complex has high enzyme activity

Added proteinSpecific enzyme activity, units/mg
Aeh1 NrdA-a/NrdA-bAeh1 NrdB
Aeh1 NrdA-a/NrdA-b0.8*94 ± 1
Aeh1 NrdB94 ± 10.3*
A. hydrophila NrdAn.a.2.4 ± 0.3
E. coli NrdAn.a.1.3 ± 0.02
E. coli NrdB0.5 ± 0.01n.a.
  • Amounts of protein used were 4 μg of Aeh1 NrdA-a/NrdA-b, Aeh1 NrdB, A. hydrophila NrdA, and E. coli NrdB, and 1 μg of E. coli NrdA. n.a., not applicable.

  • *Background enzyme activity of 4 μg of Aeh1 NrdA-a/NrdA-b, or 4 μg of Aeh1 NrdB.

  • Background activity of 4 μg of A. hydrophila NrdA was 1.8 units/mg.