Table 2.

dyf-5 separates the OSM-3 motor from IFT particles

IFT particleGenotypeMiddle segment
Distal segment
Speed, μm/snSpeed, μm/sn
KAP-1::GFPWild type0.70 ± 0.14189
osm-3 0.49 ± 0.10*91
dyf-5 0.50 ± 0.12*1100.51 ± 0.1656
OSM-3::GFPWild type0.70 ± 0.191171.14 ± 0.2343
kap-1 1.17 ± 0.24*1241.23 ± 0.2842
dyf-5 0.59 ± 0.15* 2100.59 ± 0.17* 84
dyf-5; kap-1 0.58 ± 0.15*91
CHE-11::GFPWild type0.69 ± 0.201181.20 ± 0.2749
osm-3 0.50 ± 0.09*90
dyf-5 0.49 ± 0.11*1700.49 ± 0.11*45
dyf-5; kap-1 0.60 ± 0.16*§ 1180.61 ± 0.12*§ 38
dyf-5; osm-3 0.49 ± 0.12*1090.49 ± 0.11*31
OSM-5::GFPWild type0.70 ± 0.171361.13 ± 0.2966
osm-3 0.50 ± 0.11*88
dyf-5 0.51 ± 0.11*1510.50 ± 0.11*42
dyf-5; kap-1 0.59 ± 0.19*§ 1170.58 ± 0.12*§ 45
dyf-5; osm-3 0.51 ± 0.13*103
XBX-1::GFPWild type0.70 ± 0.171411.11 ± 0.1955
dyf-5 0.50 ± 0.11*1390.47 ± 0.08*42
  • Indicated are average speeds ±SD and the number of tracks (n). Footnotes indicate significant differences when comparing speeds of: *, IFT protein in a mutant background with the same IFT protein in wild type (P < 0.001); † and ‡, OSM-3::GFP with the other IFT proteins in dyf-5(lf) animals (‡, P < 0.001; ‡, P < 0.01); and §, CHE-11 and OSM-5 in dyf-5; kap-1 with their speeds in dyf-5 and dyf-5; osm-3 animals. The speeds of all IFT proteins in dyf-5 and dyf-5; osm-3 animals, except OSM-3::GFP, were not significantly different from the speeds in osm-3 animals. Speeds in dyf-5; kap-1 animals were not significantly different from the speed of OSM-3::GFP in dyf-5 animals.