Table 1.

Analysis of hTAF4-TAFH binding, isothermal titration calorimetry binding results

Sequence sourcePeptide sequenceKd, μMn
E-box activation domain
    E2A (7–27) - AD1 [P15923]MAPVGTDKELSDLLDFSMMFP140.2 ± 4.00.802 ± 0.120
Phage display sequences
    Phage Seq 7     RWGGSDLLQTILLSAGLSGGR 5.6 ± 0.20.945 ± 0.059
    Phage Seq 7*     RWGGSDLLQTLLLSAGLSGGR 15.3 ± 0.10.885 ± 0.072
    Phage Seq 8     RGGSSDILQTAWSVAFSGGR 10.01.015
    Phage Seq 1        EWRLLHTLFPPPGGG 319.2 ± 5.60.816 ± 0.113
    Phage Seq 5     RGGSNPHMLWALFPPASGGR 606.6 ± 6.8N.D.
Candidate sequences
    Zhangfei (ZF) (71–90) [Q9NS37] YSAAEMQRFSDLLQRLLNGIGGSS 5.2 ± 2.00.810 ± 0.127
    LZIP (46–63) [AAB84166]    SDWEVDDLLCSLLSPPAS41.0 ± 5.31.003 ± 0.134
    E2F4 (366–382) [Q16254]        MSSELLEELMSSEVYAP275.50.907
    Huntingtin (1311–1328) [NP_002102]    TVCVQQLLKTLFGTNLAS>300N.D.
    HIRA (764–781) [CAG30389]     YEGGRRLLSPILLPSPISTLHSGE >300N.D.
    hSet1 (1283–1305) [O15047]SDEAERPRPLLSHILLEHNYALA>300N.D.
    PNUTS (81–100) [NP_002705]YSKTTNNIPLLQQILLTLQHSSGE >300N.D.
    HDAC9 (389–409) [AAK66821]     YNSSHQALLQHLLLKEQMRQQK>300N.D.
    PGC1α (136–155) [NP_037393]     YQEAEEPSLLKKLLLAPANTQ>360N.D.
  • A spacer sequence G-G-G or S-G-G-R, indicated in italics, was added to the C terminus of phage peptides 1, 5, 7, 7*, and 8. Other residues shown in italics were added to increase the solubility of peptides and to allow determination of the peptide concentration by UV absorbance spectroscopy. N.D., not detectable.