Table 3.

Analysis of hTAF4-TAFH binding, effects of mutations on hTAF4–TAFH binding

PeptideProteinKd, μMn
Zhangfei (71–90) [Q9NS37]hTAF4–TAFH5.2 ± 2.00.810 ± 0.127
F599R mutant19.90.917
L603E mutant11.00.617
V620R mutant10.10.667
F656R mutant13.10.713
LZIP (46–63) [AAB84166]hTAF4–TAFH41.0 ± 5.31.003 ± 0.134
F656R mutant130.81.148
  • The sequences of ZF and LZIP peptides are listed in Table 1.