Table 1.

Comparison of tumorigenic cell doses among distinct CRC phenotypic subpopulations

Lin phenotypic subpopulations*Cell doses and tumor formation
10,0008,0006,0005,0004,0002,0001,000800400200150
CD44+ 2/5
CD44 0/5
ESAhigh/CD44+ 4/517/19 13/152/516/2021/28
All the rest (mainly CD44/ESAlow)0/3 § 0/50/50/51/300/190/100/20
CD44+/CD166+ 6/10
CD44/CD166+ 1/10
CD44+/CD166 0/7
CD44/CD166 0/10
ESAhigh/CD166+ 3/5
All the rest (mainly ESAlow/CD166)0/5
ESAhigh/CD44+/CD166+ 1/2
ESAhigh/CD44+/CD166 0/2
All the rest (mainly ESAlow/CD44)0/2
  • *All populations are negative for nonepithelial lineage marker expression (Lin).

  • Cell dose, number of cells per injection; tumor formation, number of tumors formed/number of injections; tumor take was considered unsuccessful when no tumor mass was visible after 5 months follow-up. See also SI Tables 2 and 3.

  • Cell dose was 14,000.

  • §Cell dose was 12.000.

  • Five injections were performed with 2,500 cells and 14 with 2,000 cells.

  • Cells from primary tumor.