Table 1.

Cleavage by GPP of fluorogenic peptides containing the cleavage sites of glyoxysomal higher molecular weight precursor proteins

Fluorogenic peptideSequenceCleavage products
gCSAbz-EAHCV SAQ TMY*DAbz-EAH … + TMY*D
gMDHAbz-R ANCR , AK G GAY*DAbz-RANCR + ANCR + AKG + GGAY*D + GAY*D
ThiolaseAbz-SASVC , AA GDSY*DAbz-SASV … + AAGDS … + GDSY*D
ControlAbz-SASVG AAGDSY*DNone
Furin substrateAbz-RVKR GLAY*DAbz-RVKR + GLAY*D
  • Y*, (NO2)Y, m-nitro-tyrosine as quencher (acceptor); Abz, anthralinic acid as fluorogene (donor; excitation 320, emission 420 nm).

  • Predicted cleavage site.

  • Cleavage sites as identified by N-terminal sequence analysis and mass spectroscopy of the cleavage products.