Table 3.

SA inhibits the function of the kinase domain of VirA

VirA construct
0 μM AS
100 μM AS
0 μM SA10 μM SAAverage inhibition, %0 μM SA10 μM SAAverage inhibition, %
WT97 (7)70 (4)283,310 (290)2,037 (231)38
PLK2,746 (148)788 (145)716,045 (359)2,437 (326)60
LK1,080 (59)495 (27)548,682 (324)5,324 (346)39
K554 (36)263 (25)53476 (36)282 (32)41
  • A136 cells expressed virG and all virA constructs from the constitutive PN25 promoter (21). VirA domains are indicated as WT, full-length virA; PLK, amino acids 1–711; LK, amino acids 285–711; and K, amino acids 426–711. Plasmid constructs also carry a virB::lacZ fusion and have been described previously (6). Assays of vir gene expression were performed after growth in acidified induction medium containing glucose and 0 or 100 μM acetosyringone with the indicated amounts of SA. The numbers in parentheses are the SD values of three independent samples.