Table 2.

Quantitative absorption and fluorescence data of biliproteins obtained by CpeS1-catalyzed reconstitution

BiliproteinAbsorption
Fluorescence
λmax [nm] (QVis /uv)εVis (M−1·cm−1)λmax [nm]ΦF
PCB-ApcA1338/618 (4.3)111,000 (±600)6410.37 (±0.01)
PCB-ApcB344/613 (2.4)86,700 (±200)6400.20 (±0.01)
PCB-ApcA2365/622 (3.2)71,800 (±800)6410.18 (±0.01)
PCB-ApcD600 410/602 (2.5)69,000 (±500)6350.080 (±0.001)
PCB-ApcD650 346/650 (2.3)62,000 (±4,000)6630.074 (±0.002)
PCB-ApcF355/622 (2.4)96,400 (±300)6450.19 (±0.01)
PEB-CpeA(C139S)361/564 (5.8)104,400 (±700)5730.51 (±0.03)
PEB-CpeB (C48A/C59S/C165S)390/560 (5.2)120,000 (±6,000)5740.63 (±0.01)
  • Data are given for the biosynthesized and purified products, PCB-ApcA1, -ApcB, -ApcA2, -ApcD, and -ApcF; PEB-CpeA(C139S) and -CpeB(C48A/C59S/C165S). Data were averaged from two independent experiments. Reconstitutions in E. coli were done with genes from Anabaena PCC7120, except cpeAB and pebAB, which are from Calothrix PCC7601; QVis/uvdenotes the absorbance ratio of the visible and near-UV bands.