Table S2.

Cryo-EM processing and model validation

Map
C3C12_G4_12_G4_22_G4_33_G43_G4_C33in_C32in_1out1in_2out3out_C3
EMDB accession codeEMD-8784EMD-8792EMD-8787EMD-8788EMD-8789EMD-8790EMD-8791EMD-8785EMD-8783EMD-8786EMD-8793
PDB ID code5W9I5W9L5W9M5W9N5W9O5W9P5W9J5W9H5W9K
EM data processing
 No. of movie micrographs813813813813813813813813813813813
 No. of molecular projection images in map37,18037,1808,4969,3338,13311,21811,2181,98824,25010,544117
 SymmetryC3C1C1C1C1C1C3C3C1C1C3
 Map resolution (FSC 0.143, Å)3.64.04.84.75.04.54.04.84.04.611.5
 Map sharpening B-factor, Å2−88−96−127−111−43−111−93−75−94−129−81
Structure building and validationN/AN/A
 No. of atoms in deposited model28,86331,07731,08531,08532,91627,98132,91633,79832,835
 S14,0105,6555,6555,6555,6554,0105,6555,6555,642
 S23,5353,5353,5353,5353,5353,5353,5353,5353,521
 G4 VH947947947947947947947947947
 G4 VL835835835835835835835835835
 Glycans294294
 MolProbity score1.52 (95%)1.51 (95%)1.37 (98%)1.32 (98%)1.65 (91%)1.44 (96%)1.67 (90%)1.39 (97%)1.45 (96%)
 Clashscore3.813.503.122.575.952.755.703.103.68
 EMRinger score2.590.670.890.621.362.110.621.881.34
Deviations from ideal
 Bond length3 (0.02%)2 (0.01%)0 (0.00%)0 (0%)2 (0.01%)0 (0%)0 (0%)0 (0.0%)2 (0.01%)
 Bond angles93 (0.52%)147 (0.73%)136 (0.68%)126 (0.63%)142 (0.67%)75 (0.42%)108 (0.51%)127 (0.60%)122 (0.57%)
Ramachandran plot
 Favored, %95.094.796.195.995.494.594.895.895.7
 Allowed, %4.24.53.43.43.94.44.03.63.7
 Outliers, %0.90.90.60.60.71.21.20.60.6
  • N/A, not assessed.